BioTech Encyclopedia

Biofuels (algae, cellulosic, engineered microbes): uses in research

Also: biodiesel, cellulosic ethanol, isobutanol, advanced biofuels

Fuels made by living things: oil from algae, ethanol from plant fibre, and fuels from engineered yeast and E. coli.

Open questions

Gaps stated by the researchers themselves.

  • Overcoming plant cell wall recalcitrance is needed to make lignocellulose conversion cost-competitive. Himmel 2007

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Timeline

  1. 2007 Biodiesel from microalgaeWhy plant fibre is hard to turn into fuel
  2. 2008 E. coli making isobutanol
  3. 2010 E. coli making biodiesel
  4. 2012 Engineering microbes for advanced fuels

Readiness: lab lab studies · prototype demonstrated objects · commercial products or buildings in real use · review summaries of other studies.
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Start here · reviewreview biotechnology 2012

Engineering microbes for advanced fuels

Peralta-Yahya and colleagues reviewed engineering microbes, including yeast and E. coli, to make advanced biofuels.

As reported in: Peralta-Yahya, Zhang, del Cardayre et al. (2012). Microbial engineering for the production of advanced biofuels. Nature. doi:10.1038/nature11478

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biotechnology

lab biotechnology 2008

E. coli making isobutanol

Atsumi, Hanai and Liao diverted E. coli amino-acid pathway intermediates to produce higher alcohols, achieving high-yield isobutanol from glucose.

As reported in: Atsumi, Hanai, Liao (2008). Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature. doi:10.1038/nature06450

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lab biotechnology 2010

E. coli making biodiesel

Steen and colleagues engineered E. coli to make fatty esters (biodiesel), fatty alcohols and waxes directly from simple sugars.

As reported in: Steen, Kang, Bokinsky et al. (2010). Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. doi:10.1038/nature08721

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energy

review energy 2007

Biodiesel from microalgae

Chisti reviewed producing biodiesel from microalgae.

As reported in: Chisti (2007). Biodiesel from microalgae. Biotechnology Advances. doi:10.1016/j.biotechadv.2007.02.001

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review energy 2007

Why plant fibre is hard to turn into fuel

Himmel and colleagues described "biomass recalcitrance," the natural resistance of plant cell walls to breakdown, as the main reason cellulosic biofuels are costly.

As reported in: Himmel, Ding, Johnson et al. (2007). Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production. Science. doi:10.1126/science.1137016

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